PhotoSynLab

Synecho7002 tools

Neutral sites– strains with neutral sites Inducable promotor: – XylS/Pm – Ara Selection cassettes – positive selection (antibiotics) – negative selection (counterselection) : see http://2012.igem.org/Team:Edinburgh/Project/Non-antibiotic-Markers/Plac-RFP-SacB Antibiotic resistance background strains – strains with antibiotic casettes Reporter genes – GFP / YFP – Spinach Strains – Phycobilin-deficient strain – Photosystem I / II-deficient strain

Phycobilins7002

17 genes identified containing phyco* in cyanobase for Syn7002 SYNPCC7002 SYNPCC7002_A0348 definition phycobiliprotein lyase related protein, HEAT-like repeat SYNPCC7002 SYNPCC7002_A0639 definition phycocyanin-associated phycobilisome rod-core linker polypeptide SYNPCC7002 SYNPCC7002_A0811 definition Phycobilisome rod-core linker polypeptide cpcG (L-RC 28.5) SYNPCC7002 SYNPCC7002_A1631 definition allophycocyanin beta-18 subunit SYNPCC7002 SYNPCC7002_A1821 definition putative phycobilisome degradation protein SYNPCC7002 SYNPCC7002_A1928 definition allophycocyanin-associated phycobilisome 7.8-kDa core-linker polypeptide SYNPCC7002 SYNPCC7002_A1929 definition…

Neutral sites Syn7002

-> between: SYNPCC7002_A0935 and SYNPCC7002_A0936 Davies FK, Work VH, Beliaev AS, Posewitz MC (2014) Engineering Limonene and Bisabolene Production in Wild Type and a Glycogen-Deficient Mutant of Synechococcus sp. PCC 7002. Front Bioeng Biotechnol 2:21 http://www.ncbi.nlm.nih.gov/pubmed/25152894 -> glpK (SYNPCC7002_A2842) Begemann MB, Zess EK, Walters EM, Schmitt EF, Markley AL, Pfleger BF (2013) An organic acid based counter selection system for…

Lcm

LCM (=ApcE or anchor protein) Stadnichuk IN, Yanyushin MF, Bernát G, Zlenko DV, Krasilnikov PM, Lukashev EP, Maksimov EG, Paschenko VZ (2013) Fluorescence quenching of the phycobilisome terminal emitter LCM from the cyanobacterium Synechocystis sp. PCC 6803 detected in vivo and in vitro. J Photochem Photobiol B. 125:137-45 papers cited about LCM N. Adir Elucidation of the molecular structures of…

12 V pump

Brand: Reef One Product Code: 11095 The Reef One Biorb air pump, supplied by 123 Aquatics … http://123aquatics.co.uk/products/aquarium-aeration-air-pumps/biorb-12v-airpump-902.aspx little: http://www.aceofkoi.co.uk/product_info.php?manufacturers_id=52&products_id=1789#.VQ8Gh4Ut9-g big: http://www.aceofkoi.co.uk/product_info.php?manufacturers_id=52&products_id=1793#.VQ8GV4Ut9-g