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Hydrogen metabolism

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Hydrogen metabolism in cyanobacteria

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Hydrogenase genes in Synechocystis sp. PCC 6803

  • one polycistronic operon
  • sll_1220 hoxE
  • sll_1221 hoxF
  • sll_1222 hypothetical protein (nuclease?)
  • sll_1223 hoxU
  • sll_1224 hoxY (catalytic)
  • sll_1225 exonuclease ?
  • sll_1226 hoxH (catalytic)
  • slr_1876 hoxW

Literature hydrogenase

  • Carrieri D., Wawrousek K., Eckert C., Yu J., Maness P.C. (2011) The role of the bidirectional hydrogenase in cyanobacteria, Bioresource Technology, 102 (18), pp. 8368-8377.
  • Eckert C., Boehm M., Carrieri D., Yu J., Dubini A., Nixon P., Maness, P-C. (2012). Genetic analysis of the hox hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 reveals subunit roles in association, assembly, maturation, and function, J. BiOL. Chem., 287(52): 43502-43515

 Literature hydrogenase instrumentation

  •  Healey, F. P. (1970). The mechanism of hydrogen evolution by Chlamydomonas moewusii. Plant Physiology, 45, 153-159.
  • Hanus, J., Carter, K. R., & Evans, H. J. (1980). Techniques for measuring of hydrogen evolution by nodules. Methods in Enzymology, 67, 731-738.
  • Wang, R., Healey, F. P., & Myers, J. (1971). Amperiometric measurement of hydrogen evolution in Chlamydomonas. Plant Physiology, 48, 108-109.
  • Wang, R. T. (1980). Amperiometric hydrogen electrode. Methods in Enzymology, 69, 409-413.