Sum-up of pre-experiments – Anaerobic incubation of Nannochloropsis

The experiment on anaerobic incubation of Nannochloropsis will determine what anaerobic incubation does to photosynthesis, and to the production of photosynthetic lipids, storage lipids, and ω-3 fatty acids.

The pre-experiment was performed in order to find out what would be the ideal time point for making the cells anaerobic. The cells were grown in the same conditions as will be used in the main experiment (Temperature = 23 °C, light intensity = 200 μE/m²s, culture grown in 2L bottles bubbled with air).

Curves made from data recorded during the experiment

Figure 1: Cell counts vs. time. The samples were analyzed using a BD accuri c6 flow cytometer.

Figure 2: OD₆₈₀ plotted against time. A PerkinElmer Lambda 35 UV/VIS spectrometer was used for the OD measurements.

Figure 3: OD₆₈₀ plotted against time. A Tecan infinite 200 PRO multimode reader was used for the OD measurements.

Correlation curves made for cell count and OD₆₈₀

Figure 4: Correlation curve between cell counts and OD₆₈₀ for the PerkinElmer spectrometer.

Figure 5: Correlation curve between cell counts and OD₆₈₀ for the Tecan spectrometer.