Jablonski diagram

Jablonski diagram representing energy levels and spectra. Solid arrows indicate radiative transitions as occurring by absorption (violet, blue) or emission (green for fluorescence; red for phosphorescence) of a photon. Dashed arrows represent non-radiative transitions (violet, blue, green, red). Internal conversion is a non-radiative transition, which occurs when a vibrational state of a higher electronic state is coupled to a vibrational state of a lower electronic state. In the notation of, for example, S1,0, the first subscript refers to the electronic state (first excited) and the second one to the vibrational sublevel (v = 0). In the diagram the following internal conversions are indicated: S2,4→S1,0, S2,2→S1,0, S2,0→S1,0 and S1,0→S0,0. The dotted arrow from S1,0→T1,0 is a non-radiative transition called intersystem crossing, because it is a transition between states of different spin multiplicity. Below the diagram sketches of absorption-, fluorescence- and phosphorescence spectra are shown.

from: Antonie J.W.G. Visser and Olaf J. Rolinski (12/03/10) BASIC PHOTOPHYSICS

Mirror Rule

from: Olympus – Basic concepts in fluorescence

Resources

Olympus Microscopy Resource Center