|
Intersection point |
Linear correlation equation |
Correlation coefficient (R) |
||
|
Hemocytometry |
Flow cytometry |
H.cytometry |
F. cytometry |
|
|
= 0.00 |
y = 2E-08x |
y = 2E-08x |
0,9972 |
0,9728 |
|
≠ 0.00 |
y = 2E-08x + 0,0024 |
y = 2E-08x + 0,0047 |
0,9986 |
0,9808 |
The correlation equations are then used to determine the cell number of your cell culture, where x is the cell number in the cell culture, and y is the OD750nm value of that cell culture. The difference between the equations are not significant either the correlation line goes trough the intersection point equal zero, or not. Therefore, both can be used.