http://chimera.labs.oreilly.com/books/1234000002013/index.html
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Sulfur in Cyanobacteria
https://www.dropbox.com/s/aouapvzoej3sac2/Aklujkar%282013%29_Microbiol.pdf?dl=0 Papers with this topic: L.J. STAL and W.E. KRUMBEIN (1984) METABOLISM OF CYANOBACTERIA IN ANAEROBIC MARINE SEDIMENTSGERBAM — Deuxième Colloque International de Bactériologie marine — CNRS, Brest, 1-5 octobre 1984 1FREMER, Actes de Colloques, 3, 1986, pp. 301-309 Reference provided by Niels-Ulrik Frigaard. This paper cites the two papers below: Sheridan RP, Castenholz RW (1968) Production of hydrogen sulphide…
Conferences 2015
Swedish Consortium for Artificial Photosynthesis April 26th – May 1st, Uppsala, Sweden ComBio 2015 September 27 – October 1st, Melbourne
Antibiotic Susceptibility of Nannochloropsis strains
1.Cell cultures of N. oceanica and N. gaditana were grown in the Cell-Hi CN medium at temperature 23°C with constant illumination at 100 μE m−2 s−1 and constant shaking on a Heidolph® Rotamax 120 Orbital Shaker (see Chapter II – Materials and methods – Growth conditions). 2.Agar plates of 9 cm diameter were used for the plating out of the cell culture. The…
Photosynthetic Machinery Nannochloropsis
Search 2014_08_21: Nannochloropsis & Photosystem Fisher(1996)_JPlanktRes.pdf Braun(2014)_JPhycol.pdf Szabo(2014)_BioreTech.pdf Basso(2014)_BiochimBiophysActa (Case Conflict).pdf
Biolistic transformation of Nannochloropsis oceanica CCMP1779
The transformation was performed with pLit_chlL_chlor transformation plasmid containing chloramphenicol resistance cassette to delete the endogenous chlL gene (chloroplast genome) of Nannochlropsis oceanica. Cell culture The cells of N. oceanica CCMP1779 were cultivated in the Cell-Hi CN medium at 23°C at constant light intensity of 100 µE m-2 s-1 and continuous rotational shaking. After reaching the exponential growth phase concentration…
Biolistic transformation (microparticle bombardment)
Biolistic_PDS1000 manual
Electroporation of Nannochloropsis oceanica CCMP1779
The transformation of the Nannochloropsis oceanica cells by electroporation was performed using the pSELECT100 transformation plasmid containing the hygromycin resistance cassette. Nannochloropsis oceanica CCMP1779 grew to a cell density of 3-8×106 cells/mL (competent cells). A total of 1-5×106 cells were harvested by centrifugation (3700 × g) for 10-15 min at 4°C. Collected cells were washed 2-3 times with 375 mM…