PhotoSynLab

Author: martin_hm

Molecular tools – BT8105

PCR invention   Different types of DNA polymerases https://www.neb.com/tools-and-resources/selection-charts/dna-polymerase-selection-chart   Restriction Enzymes   Golden Gate cloning Gibson cloning   TALEN Methylation in Bacteria https://www.neb.com/tools-and-resources/selection-charts/dam-dcm-and-cpg-methylation   BioBricks   Biopython Bioperl   Bioconductor     DNA synthesis   from: http://synbiobeta.com/     Benchling   VisualBiologyLanguage (VisBOL) http://visbol.org   SBOL / PidgenCad http://pigeoncad.org/ http://sbolstandard.org/software/tools/   Databases of relevance: https://en.wikipedia.org/wiki/List_of_biological_databases   Resistance fusion genes…

Group Meeting, Feb 16th, 2017

Time: 13:00-14:00 Room: R Botanikk 2 (360.D1-161)   Olha gave a presentation …   In vivo cloning (Rahmi) The first article reporting in vivo cloning method is: Rapid cloning by homologous recombination in vivo. Bubeck et al 1993. https://doi.org/10.1093/nar/21.15.3601 And a recent extension of the method is: Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with…

Chloroplast transformation

Potential genes for deletion These genes are not essential components of PSII in Synechocystis psbX psbY psbZ These genes are involved in the light-independent chlorophyll synthesis chlL chlN [wpfilebase tag=file id=333 tpl=simple /] information concerning light-dependent and light independent chlorophyll synthesis Potential neutral region in chloroplast genome The region is downstream of atpB in the Chlamydomonas chloroplast genome (see Purton(2007)_ToolsTechChloroTrans).…