PhotoSynLab

Molecular tools – BT8105

PCR invention

 

Different types of DNA polymerases

https://www.neb.com/tools-and-resources/selection-charts/dna-polymerase-selection-chart

 

Restriction Enzymes

 

Golden Gate cloning

Gibson cloning

 

TALEN

Methylation in Bacteria

https://www.neb.com/tools-and-resources/selection-charts/dam-dcm-and-cpg-methylation

 

BioBricks

 

Biopython

Bioperl

 

Bioconductor

 

 

DNA synthesis

Carlson_Price_Seq_Synth_Feb2014-thumb-500x3981

 

Carlson_Price_sDNA_Feb2014-thumb-500x4111

from: http://synbiobeta.com/

 

 

Benchling

 

VisualBiologyLanguage (VisBOL)

http://visbol.org

 

SBOL / PidgenCad

http://pigeoncad.org/

http://sbolstandard.org/software/tools/

 

Databases of relevance:

https://en.wikipedia.org/wiki/List_of_biological_databases

 

Resistance fusion genes

database: https://ardb.cbcb.umd.edu/

 

GFP-zeocin

http://www.biotechniques.com/multimedia/archive/00007/98243pf01_7619a.pdf

 

beta-lactamase

Zlokarnik, G. 2000. Fusions to beta-lactamase as a reporter for gene expression in live mammalian cells. Methods Enzymol. 326:221-244

 

chloramphenicol acetyltransfereases

https://www.ncbi.nlm.nih.gov/pubmed/10649231

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178582/

http://link.springer.com/protocol/10.1385/1-59259-396-8%3A45#page-1

 

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