PhotoSynLab

Changes in lipid composition induced by environmental and genetic manipulation

We will investigate whether oxygen depletion will change the lipid composition in Synechocystis sp. PCC 6803 using chromatography and mass spectrometry techniques. Several enzymes with homology to enzymes involved in ubiquinone biosynthesis in other organisms appear to be involved in plastoquinone synthesis in cyanobacteria. We will investigate the functions of open reading frames with homology to ubiquinone synthesis in other…

SacB casette

Bacterial levansucrases synthesize high molecular weight, β-(2,6)-linked levan from sucrose by transfer of fructosyl units. This polymer is toxic to cyanobacteria. Literature: gene http://dev.sbkb.org/kb-shared-files/2009ETW/Posters/ET_09_Wrobel_poster.pdf Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli. Gay P, Le Coq D, Steinmetz M, Ferrari E, Hoch JA. J Bacteriol. 1983 Mar;153(3):1424-31. The DNA…

Synechocystis Antibiotic cassettes

The following table provide information on the origin of resistance cassettes in Synechocystis sp. PCC 6803. Vermaas(1996)_JAppPhycol.pdf [wpfilebase tag=file id=4 tpl=simple /] [wpfilebase tag=fileurl id=1 linktext=’EatonRye PhotosynthMeth’ /] [wpfilebase tag=file id=3 tpl=download-button /] Source of chloramphenicol, erythromycin, kanamycin and spectinomycin.   Eaton-Rye(2011)_PhotosynthResProt.pdf Source of chloramphenicol, erythromycin, kanamycin and spectinomycin. Eaton-Rye(2004)_PhotosynthResProt.pdf Source of chloramphenicol, erythromycin, kanamycin and spectinomycin. [wpfilebase tag=list id=2…