1. 47-mm glass fiber paper filters (Whatman GF/C, Schleicher & Schuell Co.) were presaturated with 100 ml distilled water and dried overnight at 60°C and 1h at 120°C, to evaporate all the water. Eppendorf tube containing a presaturated paper filter were pre- weighted. 2. Replicate aliquots (10 ml) of culture suspensions were centrifuged (4700xg, 10 min, room temperature). 3. washed with 80…
Author: admin
Publication – Congratulations Alice and Gunvor!
8 November 2013 A manuscript authored by Alice and Gunvor has been accepted for publication: A. Mühlroth, K. Lee, G Røkke, P. Winge, Y. Olsen, M.F. Hohmann-Marriott, O. Vadstein & A.M. Bones Pathways of lipid metabolism in marine algae, co-expression network, bottlenecks and candidate genes for enhanced production of EPA and DHA in species of Chromista. Marine Drugs doi:10.3390/md11114662 Congratulations Alice…
Conjugation protocol for Psychrophilic Pseudomonas sp.
* 3 ml pre-cultures of E. coli S17_1 and psychrophilic Pseudomonas sp. (or Pseudomonas putida instead or psychrophilic Pseudomonas) were used to inoculate an appropriate amount of LB to perform conjugation. * E. coli was incubated for 4 h, and psychrophilic Pseudomonas sp. (or Pseudomonas putida) were incubated for 5 h. * 2 ml E. coli S17_1 which contains…
Changes in lipid composition induced by environmental and genetic manipulation
We will investigate whether oxygen depletion will change the lipid composition in Synechocystis sp. PCC 6803 using chromatography and mass spectrometry techniques. Several enzymes with homology to enzymes involved in ubiquinone biosynthesis in other organisms appear to be involved in plastoquinone synthesis in cyanobacteria. We will investigate the functions of open reading frames with homology to ubiquinone synthesis in other…
developed conjugation protocol
The protocol used [wpfilebase tag=file id=22 tpl=simple /] [wpfilebase tag=file id=23 tpl=simple /] to develop conjugation protocol
First platereading
Two centrifuge tubes (50 ml) where filled with media and algaecells (Nannochloropsis CCMP 1779) that had been growing for 3 days. The tubes where then centrifuged at 6000 rpm for 5 minutes, and some of the supernatant was taken away. This was done to concentrate the cells approximately 8-folds. The solution was then transferred to a 24-well plate to make…
Working with the spectrophotometer.
We took cells from agar plates and diluted them with water. The spectra was then obtained using a spectrophotometer (infinite M200PRO). The data was saved at the lab computer as SpecScan1.xlsx. The cuvettes containing Synne6803 (1:10) and Nanno1779 (1:20) was put in a refridgerator for later color comparison, as the color they had showed a good absorption level for measurements.…