Intersection point Linear correlation equation Correlation coefficient (R) Hemocytometry Flow cytometry H.cytometry F. cytometry = 0.00 y = 2E-08x y = 2E-08x 0,9972 0,9728 ≠ 0.00 y = 2E-08x + 0,0024 y = 2E-08x + 0,0047 0,9986 0,9808 The correlation equations are then used to determine the cell number of your cell culture, where x is the cell number in…
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Flow cytometer (setups)
Running the flow cytometer BD Accuri C6 Choose between the number of events counted or velocity the cell culture is drawn through the flow cytometer Threshold – indicates which cells will be excluded from a count Wash cycles (miliQ water) – washing the nozzle Agitation cycles – agitation of the cell samples Fluorescence filters – 4 different FL1 533 ± 15…
Publication – Congratulations Jacob!
30 July 2014 A manuscript authored by Jacob has been accepted for publication: Jacob J. Lamb, Ryan E. Hill, Julian J. Eaton-Rye, Martin F. Hohmann-Marriott Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803 PlosOne Congratulations Jacob!
Graduation Ceremony for Master Students
The Master Students of 2014!
From the left: Axana Chernyavskaya, Kristin Forfang, Martin Hohmann-Marriott (Assoc. Prof.), Binod Gautam and Benedikte Bøe
Book published
May 4, 2014 Martin’s book has been published in the “Advances in Photosynthesis and Respiration” series:
Fluorescein
Evaluated addition of fluorescein to Synechocystis cells for standardizing 77K fluorescence spectra (excited at 435 nm). Measured absorption at peak 490nm: 0.53 stock solution was diluted 1:3 -> corrected absorption for stock solution at peak 2.12 from: https://en.wikipedia.org/wiki/Fluorescein Extinction coefficient at 490 nm = 76900 Mol-1 cm-1 Stock solution concentration: A=e*L*C C=A/(e*L) C=2.12 / (76900 cm-1 M * 1 cm…
Absorption and Fluorescence
Jablonski diagram Jablonski diagram representing energy levels and spectra. Solid arrows indicate radiative transitions as occurring by absorption (violet, blue) or emission (green for fluorescence; red for phosphorescence) of a photon. Dashed arrows represent non-radiative transitions (violet, blue, green, red). Internal conversion is a non-radiative transition, which occurs when a vibrational state of a higher electronic state is coupled to…
Absorption & Fluorescence spectra Synecho
Whole Cell Absorption 77K [wpfilebase tag=file id=330 tpl=simple /] Thylakoid and purified PSII 77K fluorescence [wpfilebase tag=file id=329 tpl=simple /] A: Excitation at 600 nm excites the phycobilisome and preferentially PSII B: Excitation at 420 nm excites the chlorophylls contained in PSI and PSII PSI , PSI-IsiA and IsiA fluorescence at 77K [wpfilebase tag=file id=328 tpl=simple /]
Cryopreservation of marine algae, Nannochloropsis
1 cryopreservation Cultivate N. oceanica or N. gaditana on a shaker (150 rpm, 23°C, 100 yE) to a cell concentration of 1*107 cells/ml. Place culture for 20 hours in the dark before cryopreservation. Equilibrate the CoolCell freezing container (check if black core is inside) at 4°C. Prepare a sterile filtered 20% solution of DMSO in the culture medium used to grow the algae. harvest the…
determination of particulate carbon and nitrogen
1. Take replicate samples (2-5 ml) of the cell culture and measure the optical density. (for Nannochloropsis OD at 750 nm). 2. Wash equipment with 10% HCl and set it up as followed: Use an Erlenmeyer flask connected to a vacuum pump, place a rubber stopper with a filter inserted in the middle of it, on the Erlenmeyer flask. 3.…