Synechocystis [wpfilebase tag=file id=21 tpl=simple /]
Category: Uncategorised
Micro_titer_plates
Paper on basics for measuring growth (liquid+solid) in micro titer plates: [wpfilebase tag=file id=16 tpl=simple /]
MnO2 / manganese dioxide
[wpfilebase tag=file id=15 tpl=simple /] [wpfilebase tag=file id=14 tpl=simple /]
Gold
Article concerning the accumulation of gold by bacteria: [wpfilebase tag=file id=10 tpl=simple /] [wpfilebase tag=file id=20 tpl=simple /] [wpfilebase tag=file id=20 tpl=download-button /]
Changes in lipid composition induced by environmental and genetic manipulation
We will investigate whether oxygen depletion will change the lipid composition in Synechocystis sp. PCC 6803 using chromatography and mass spectrometry techniques. Several enzymes with homology to enzymes involved in ubiquinone biosynthesis in other organisms appear to be involved in plastoquinone synthesis in cyanobacteria. We will investigate the functions of open reading frames with homology to ubiquinone synthesis in other…
SacB casette
Bacterial levansucrases synthesize high molecular weight, β-(2,6)-linked levan from sucrose by transfer of fructosyl units. This polymer is toxic to cyanobacteria. Literature: gene http://dev.sbkb.org/kb-shared-files/2009ETW/Posters/ET_09_Wrobel_poster.pdf Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli. Gay P, Le Coq D, Steinmetz M, Ferrari E, Hoch JA. J Bacteriol. 1983 Mar;153(3):1424-31. The DNA…
Synechocystis Antibiotic cassettes
The following table provide information on the origin of resistance cassettes in Synechocystis sp. PCC 6803. Vermaas(1996)_JAppPhycol.pdf [wpfilebase tag=file id=4 tpl=simple /] [wpfilebase tag=fileurl id=1 linktext=’EatonRye PhotosynthMeth’ /] [wpfilebase tag=file id=3 tpl=download-button /] Source of chloramphenicol, erythromycin, kanamycin and spectinomycin. Eaton-Rye(2011)_PhotosynthResProt.pdf Source of chloramphenicol, erythromycin, kanamycin and spectinomycin. Eaton-Rye(2004)_PhotosynthResProt.pdf Source of chloramphenicol, erythromycin, kanamycin and spectinomycin. [wpfilebase tag=list id=2…
developed conjugation protocol
The protocol used [wpfilebase tag=file id=22 tpl=simple /] [wpfilebase tag=file id=23 tpl=simple /] to develop conjugation protocol
First platereading
Two centrifuge tubes (50 ml) where filled with media and algaecells (Nannochloropsis CCMP 1779) that had been growing for 3 days. The tubes where then centrifuged at 6000 rpm for 5 minutes, and some of the supernatant was taken away. This was done to concentrate the cells approximately 8-folds. The solution was then transferred to a 24-well plate to make…
Working with the spectrophotometer.
We took cells from agar plates and diluted them with water. The spectra was then obtained using a spectrophotometer (infinite M200PRO). The data was saved at the lab computer as SpecScan1.xlsx. The cuvettes containing Synne6803 (1:10) and Nanno1779 (1:20) was put in a refridgerator for later color comparison, as the color they had showed a good absorption level for measurements.…