These antibiotics have been evaluated as selection markers. The used concentration is shown in brackets. Evaluated by: [wpfilebase tag=file id=130 tpl=simple /] [wpfilebase tag=file id=134 tpl=simple /] zeocin (2 μg / mL) blasticidin S (50 μg / mL) hygromycin B (300 μg / mL) Evaluated by: [wpfilebase tag=file id=131 tpl=simple /] zeocin (3 μg / mL) Evaluated by: [wpfilebase…
Category: Uncategorised
C2 metabolism in cyanobacteria – photorespiration
Figure source: [wpfilebase tag=file id=129 tpl=simple /] The 2-phosphoglycolate metabolism in cyanobacteria comprises three routes: – the canonical C2 cycle (Plant and cyanobacteria) – the glycerate pathway (cyanobacteria – specific) – decarboxylation path (cyanobacteria – specific) ♦ Plant C2 cycle enzymes of cyanobacterial origin are shown in blue ♦ Enzymes originating from proteobacteria are shown in red. ♦ Enzymes shown in…
determination of particulate carbon and nitrogen
1. Take replicate samples (2-5 ml) of the cell culture and measure the optical density. (for Nannochloropsis OD at 750 nm). 2. Wash equipment with 10% HCl and set it up as followed: Use an Erlenmeyer flask connected to a vacuum pump, place a rubber stopper with a filter inserted in the middle of it, on the Erlenmeyer flask. 3.…
determination of the dry weight
1. 47-mm glass fiber paper filters (Whatman GF/C, Schleicher & Schuell Co.) were presaturated with 100 ml distilled water and dried overnight at 60°C and 1h at 120°C, to evaporate all the water. Eppendorf tube containing a presaturated paper filter were pre- weighted. 2. Replicate aliquots (10 ml) of culture suspensions were centrifuged (4700xg, 10 min, room temperature). 3. washed with 80…
Protein expression cyanobacteria
literature [wpfilebase tag=file id=91 tpl=simple /]
Hydrogen metabolism
Hydrogen metabolism in cyanobacteria [wpfilebase tag=file id=96 tpl=simple /] [wpfilebase tag=file id=95 tpl=simple /] [wpfilebase tag=file id=94 tpl=simple /] [wpfilebase tag=file id=93 tpl=simple /] [wpfilebase tag=file id=92 tpl=simple /] [wpfilebase tag=file id=90 tpl=simple /] Hydrogenase genes in Synechocystis sp. PCC 6803 one polycistronic operon sll_1220 hoxE sll_1221 hoxF sll_1222 hypothetical protein (nuclease?) sll_1223 hoxU sll_1224 hoxY (catalytic) sll_1225 exonuclease ?…
Conjugation protocol for Psychrophilic Pseudomonas sp.
* 3 ml pre-cultures of E. coli S17_1 and psychrophilic Pseudomonas sp. (or Pseudomonas putida instead or psychrophilic Pseudomonas) were used to inoculate an appropriate amount of LB to perform conjugation. * E. coli was incubated for 4 h, and psychrophilic Pseudomonas sp. (or Pseudomonas putida) were incubated for 5 h. * 2 ml E. coli S17_1 which contains…
Genome of Nannochloropsis and related algae
Link to chloroplast genomes (including Nannochloropsis species) http://www.ebi.ac.uk/genomes/organelle.html Origin of chloroplast [wpfilebase tag=file id=39 tpl=simple] [wpfilebase tag=file id=48 tpl=simple] Chloroplast and mitochondrion genomes [wpfilebase tag=file id=41 tpl=simple] [wpfilebase tag=file id=40 tpl=simple] Evolution of haptophytes and cryptophytes [wpfilebase tag=file id=46 tpl=simple] [wpfilebase tag=file id=47 tpl=simple]
Literature: Utilization of FeIII and MnIV
[wpfilebase tag=file id=38 tpl=simple /] Abstract Whole-genome microarray analysis of Geobacter sulfurreducens grown on insoluble Fe(III) oxide or Mn(IV) oxide versus soluble Fe(III) citrate revealed significantly different expression patterns. The most upregulated genes, omcS and omcT, encode cell-surface c-type cytochromes, OmcS being required for Fe(III) and Mn(IV) oxide reduction. Other electron transport genes upregulated on both metal oxides included genes…