1.Cell cultures of N. oceanica and N. gaditana were grown in the Cell-Hi CN medium at temperature 23°C with constant illumination at 100 μE m−2 s−1 and constant shaking on a Heidolph® Rotamax 120 Orbital Shaker (see Chapter II – Materials and methods – Growth conditions). 2.Agar plates of 9 cm diameter were used for the plating out of the cell culture. The…
22 August 2014 Axana Chernyavskaya concluded her Master thesis today and presented her work: Developing molecular tools for the genetic manipulation of Nannochloropsis Congratulations on a job well done!
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The transformation was performed with pLit_chlL_chlor transformation plasmid containing chloramphenicol resistance cassette to delete the endogenous chlL gene (chloroplast genome) of Nannochlropsis oceanica. Cell culture The cells of N. oceanica CCMP1779 were cultivated in the Cell-Hi CN medium at 23°C at constant light intensity of 100 µE m-2 s-1 and continuous rotational shaking. After reaching the exponential growth phase concentration…
Biolistic_PDS1000 manual
The transformation of the Nannochloropsis oceanica cells by electroporation was performed using the pSELECT100 transformation plasmid containing the hygromycin resistance cassette. Nannochloropsis oceanica CCMP1779 grew to a cell density of 3-8×106 cells/mL (competent cells). A total of 1-5×106 cells were harvested by centrifugation (3700 × g) for 10-15 min at 4°C. Collected cells were washed 2-3 times with 375 mM…
19.08.2014 Kristin and Benedikte successfully concluded their Master’s Thesis examinations today. Congratulations for a job well done! Kristin Forfang: Changes in Lipid Composition induced by environmental changes and genetic manipulation in Synechocystis sp. PCC 6803 Benedikte Bøe: Investigation of the function of pilin genes in the cyanobacterium Synechocystis sp. PCC 6803
Intersection point Linear correlation equation Correlation coefficient (R) Hemocytometry Flow cytometry H.cytometry F. cytometry = 0.00 y = 2E-08x y = 2E-08x 0,9972 0,9728 ≠ 0.00 y = 2E-08x + 0,0024 y = 2E-08x + 0,0047 0,9986 0,9808 The correlation equations are then used to determine the cell number of your cell culture, where x is the cell number in…